The evolution of parasitic and mutualistic plant-virus symbioses through transmission-virulence trade-offs.

Virus-plant interactions range from parasitism to mutualism. Viruses have been shown to increase fecundity of infected plants in comparison with uninfected plants under certain environmental conditions. Increased fecundity of infected plants may benefit both the plant and the virus as seed transmission is one of the main virus transmission pathways, in addition to vector transmission. Trade-offs between vertical (seed) and horizontal (vector) transmission pathways may involve virulence, defined here as decreased fecundity in infected plants. To better understand plant-virus symbiosis evolution, we explore the ecological and evolutionary interplay of virus transmission modes when infection can lead to an increase in plant fecundity. We consider two possible trade-offs: vertical seed transmission vs infected plant fecundity, and horizontal vector transmission vs infected plant fecundity (virulence). Through mathematical models and numerical simulations, we show (1) that a trade-off between virulence and vertical transmission can lead to virus extinction during the course of evolution, (2) that evolutionary branching can occur with subsequent coexistence of mutualistic and parasitic virus strains, and (3) that mutualism can out-compete parasitism in the long-run. In passing, we show that ecological bi-stability is possible in a very simple discrete-time epidemic model. Possible extensions of this study include the evolution of conditional (environment-dependent) mutualism in plant viruses

Underground Environment Aware MIMO Design Using Transmit and Receive Beamforming in Internet of Underground Things

In underground (UG) multiple-input and multiple-output (MIMO), the transmit beamforming is used to focus energy in the desired direction. There are three different paths in the underground soil medium through which the waves propagates to reach at the receiver. When the UG receiver receives a desired data stream only from the desired path, then the UG MIMO channel becomes three path (lateral, direct, and reflected) interference channel. Accordingly, the capacity region of the UG MIMO three path interference channel and degrees of freedom (multiplexing gain of this MIMO channel requires careful modeling). Therefore, expressions are required derived the degrees of freedom of the UG MIMO interference channel. The underground receiver needs to perfectly cancel the interference from the three different components of the EM-waves propagating in the soil medium. This concept is based upon reducing the interference the undesired components to minimum at UG receiver using the receive beamforming. In this paper, underground environment aware MIMO using transmit and receive beamforming has been developed. The optimal transmit beamforming and receive combin- ing vectors under minimal inter-component interference constraint are derived. It is shown that UG MIMO performs best when all three component of the wireless UG channel are leveraged for beamforming. The environment aware UG MIMO technique leads to three-fold performance improvements and paves the wave for design and development of next generation sensor-guided irrigation systems in the field of digital agriculture

Effect of a ring-shaped laser beam on the weldability of aluminum-to-hilumin for battery tab connectors

Advances in laser beam shaping technologies are being studied and are considered beneficial in many aspects of dissimilar metal joining, which include reducing intermetallic compounds (IMCs), optimizing weld pool profiles, and controlling porosity and spatters. This paper utilizes a coaxial ring and core dual beam laser and aims to study the impact of the power ratios between core and ring beams on the weldability of 1100 aluminum alloy to hilumin (steel). High-resolution electron microscopy was performed in the cross sections of the weld pools to quantify the melt pool composition and subsequent IMC formation and weld defects (cracking and cavitation). Lap-shear mechanical testing and electrical resistivity testing were also carried out. Results showed that the optimal power ratio for lap-shear strength was 0.4 (intermediate core and ring) due to the reduction in the Fe-rich liquid into the upper weld region. As a result, this produced a smaller interface between the Fe-rich region and Al, thus reducing the formation of the most detrimental IMC (e.g., Fe2Al5). Conversely, a power ratio of 0.2 (core-dominant) was found beneficial for reducing electrical resistance due to a reduced total IMC volume

A duplication at chromosome 11q12.2–11q12.3 is associated with spinocerebellar ataxia type 20

Spinocerebellar ataxia type 20 (SCA20) has been linked to chromosome 11q12, but the underlying genetic defect has yet to be identified. We applied single-nucleotide polymorphism genotyping to detect structural alterations in the genomic DNA of patients with SCA20. We found a 260 kb duplication within the previously linked SCA20 region, which was confirmed by quantitative polymerase chain reaction and fiber fluorescence in situ hybridization, the latter also showing its direct orientation. The duplication spans 10 known and 2 unknown genes, and is present in all affected individuals in the single reported SCA20 pedigree. While the mechanism whereby this duplication may be pathogenic remains to be established, we speculate that the critical gene within the duplicated segment may be DAGLA, the product of which is normally present at the base of Purkinje cell dendritic spines and contributes to the modulation of parallel fiber-Purkinje cell synapses

“Structuration” by intellectual organization: the configuration of knowledge in relations among structural components in networks of science

Using aggregated journal–journal citation networks, the measurement of the knowledge base in empirical systems is factor-analyzed in two cases of interdisciplinary developments during the period 1995–2005: (i) the development of nanotechnology in the natural sciences and (ii) the development of communication studies as an interdiscipline between social psychology and political science. The results are compared with a case of stable development: the citation networks of core journals in chemistry. These citation networks are intellectually organized by networks of expectations in the knowledge base at the specialty (that is, above-journal) level. The “structuration” of structural components (over time) can be measured as configurational information. The latter is compared with the Shannon-type information generated in the interactions among structural components: the difference between these two measures provides us with a measure for the redundancy generated by the specification of a model in the knowledge base of the system. This knowledge base incurs (against the entropy law) to variable extents on the knowledge infrastructures provided by the observable networks of relations

On the Area Requirements of Planar Greedy Drawings of Triconnected Planar Graphs

In this paper we study the area requirements of planar greedy drawings of triconnected planar graphs. Cao, Strelzoff, and Sun exhibited a family $\cal H$ of subdivisions of triconnected plane graphs and claimed that every planar greedy drawing of the graphs in $\mathcal H$ respecting the prescribed plane embedding requires exponential area. However, we show that every $n$-vertex graph in $\cal H$ actually has a planar greedy drawing respecting the prescribed plane embedding on an $O(n)\times O(n)$ grid. This reopens the question whether triconnected planar graphs admit planar greedy drawings on a polynomial-size grid. Further, we provide evidence for a positive answer to the above question by proving that every $n$-vertex Halin graph admits a planar greedy drawing on an $O(n)\times O(n)$ grid. Both such results are obtained by actually constructing drawings that are convex and angle-monotone. Finally, we consider $\alpha$-Schnyder drawings, which are angle-monotone and hence greedy if $\alpha\leq 30^\circ$, and show that there exist planar triangulations for which every $\alpha$-Schnyder drawing with a fixed $\alpha<60^\circ$ requires exponential area for any resolution rule

Wolbachia and DNA barcoding insects: patterns, potential and problems

Wolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts. Wolbachia can confuse the patterns of mitochondrial variation, including DNA barcodes, because it influences the pathways through which mitochondria are inherited. We examined the extent to which these endosymbionts are detected in routine DNA barcoding, assessed their impact upon the insect sequence divergence and identification accuracy, and considered the variation present in Wolbachia COI. Using both standard PCR assays (Wolbachia surface coding protein – wsp), and bacterial COI fragments we found evidence of Wolbachia in insect total genomic extracts created for DNA barcoding library construction. When >2 million insect COI trace files were examined on the Barcode of Life Datasystem (BOLD) Wolbachia COI was present in 0.16% of the cases. It is possible to generate Wolbachia COI using standard insect primers; however, that amplicon was never confused with the COI of the host. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. We conclude that the presence of the Wolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library; in fact, the ability to query this DNA library (the database and the extracts) for endosymbionts is one of the ancillary benefits of such a large scale endeavor – for which we provide several examples. It is our conclusion that regular assays for Wolbachia presence and type can, and should, be adopted by large scale insect barcoding initiatives. While COI is one of the five multi-locus sequence typing (MLST) genes used for categorizing Wolbachia, there is limited overlap with the eukaryotic DNA barcode region

Forecasting daily attendances at an emergency department to aid resource planning

<p>Abstract</p> <p>Background</p> <p>Accurate forecasting of emergency department (ED) attendances can be a valuable tool for micro and macro level planning.</p> <p>Methods</p> <p>Data for analysis was the counts of daily patient attendances at the ED of an acute care regional general hospital from July 2005 to Mar 2008. Patients were stratified into three acuity categories; i.e. P1, P2 and P3, with P1 being the most acute and P3 being the least acute. The autoregressive integrated moving average (ARIMA) method was separately applied to each of the three acuity categories and total patient attendances. Independent variables included in the model were public holiday (yes or no), ambient air quality measured by pollution standard index (PSI), daily ambient average temperature and daily relative humidity. The seasonal components of weekly and yearly periodicities in the time series of daily attendances were also studied. Univariate analysis by t-tests and multivariate time series analysis were carried out in SPSS version 15.</p> <p>Results</p> <p>By time series analyses, P1 attendances did not show any weekly or yearly periodicity and was only predicted by ambient air quality of PSI > 50. P2 and total attendances showed weekly periodicities, and were also significantly predicted by public holiday. P3 attendances were significantly correlated with day of the week, month of the year, public holiday, and ambient air quality of PSI > 50.</p> <p>After applying the developed models to validate the forecast, the MAPE of prediction by the models were 16.8%, 6.7%, 8.6% and 4.8% for P1, P2, P3 and total attendances, respectively. The models were able to account for most of the significant autocorrelations present in the data.</p> <p>Conclusion</p> <p>Time series analysis has been shown to provide a useful, readily available tool for predicting emergency department workload that can be used to plan staff roster and resource planning.</p

Quasi-Normal Modes of Stars and Black Holes

Perturbations of stars and black holes have been one of the main topics of relativistic astrophysics for the last few decades. They are of particular importance today, because of their relevance to gravitational wave astronomy. In this review we present the theory of quasi-normal modes of compact objects from both the mathematical and astrophysical points of view. The discussion includes perturbations of black holes (Schwarzschild, Reissner-Nordstr\"om, Kerr and Kerr-Newman) and relativistic stars (non-rotating and slowly-rotating). The properties of the various families of quasi-normal modes are described, and numerical techniques for calculating quasi-normal modes reviewed. The successes, as well as the limits, of perturbation theory are presented, and its role in the emerging era of numerical relativity and supercomputers is discussed.Comment: 74 pages, 7 figures, Review article for "Living Reviews in Relativity